LymphedemaV1, Main, Exploration, bibRecord, 000C68

Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity

Identifieur interne : 000C68 ( Main/Exploration ); précédent : 000C67; suivant : 000C69

Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity

Auteurs : Timothy Kassis [Géorgie (pays)] ; Sri Charan Yarlagadda [Géorgie (pays)] ; Alison B. Kohan [États-Unis] ; Patrick Tso [États-Unis] ; Victor Breedveld [Géorgie (pays)] ; J. Brandon Dixon [Géorgie (pays)]

Source :

American Journal of Physiology - Gastrointestinal and Liver Physiology [ 0193-1857 ] ; 2016.

RBID : PMC:4888550

Abstract

Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, the acute lymphatic pump response in the mesentery to a postprandial lipid meal has gone unexplored. In this study, we used the rat mesenteric collecting vessel as an in vivo model to quantify the effect of lipoproteins on vessel function. Lipid load was continuously monitored by using the intensity of a fluorescent fatty-acid analog, which we infused along with a fat emulsion through a duodenal cannula. The vessel contractility was simultaneously quantified. We demonstrated for the first time that collecting lymphatic vessels respond to an acute lipid load by reducing pump function. High lipid levels decreased contraction frequency and amplitude. We also showed a strong tonic response through a reduction in the end-diastolic and systolic diameters. We further characterized the changes in flow rate and viscosity and showed that both increase postprandially. In addition, shear-mediated Ca²⁺ signaling in lymphatic endothelial cells differed when cultured with lipoproteins. Together these results show that the in vivo response could be both shear and lipid mediated and provide the first evidence that high postprandial lipid has an immediate negative effect on lymphatic function even in the acute setting.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888550

DOI: 10.1152/ajpgi.00318.2015
PubMed: 26968208
PubMed Central: 4888550

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, the acute lymphatic pump response in the mesentery to a postprandial lipid meal has gone unexplored. In this study, we used the rat mesenteric collecting vessel as an in vivo model to quantify the effect of lipoproteins on vessel function. Lipid load was continuously monitored by using the intensity of a fluorescent fatty-acid analog, which we infused along with a fat emulsion through a duodenal cannula. The vessel contractility was simultaneously quantified. We demonstrated for the first time that collecting lymphatic vessels respond to an acute lipid load by reducing pump function. High lipid levels decreased contraction frequency and amplitude. We also showed a strong tonic response through a reduction in the end-diastolic and systolic diameters. We further characterized the changes in flow rate and viscosity and showed that both increase postprandially. In addition, shear-mediated Ca<sup>2+</sup>
 signaling in lymphatic endothelial cells differed when cultured with lipoproteins. Together these results show that the in vivo response could be both shear and lipid mediated and provide the first evidence that high postprandial lipid has an immediate negative effect on lymphatic function even in the acute setting.</p>
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Serveur d'exploration sur le lymphœdème

Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity

Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity

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